适体
化学
检出限
荧光
线性范围
临床诊断
组合化学
劈理(地质)
联轴节(管道)
纳米技术
生物物理学
分子生物学
色谱法
医学
机械工程
物理
材料科学
量子力学
生物
工程类
临床心理学
岩土工程
断裂(地质)
作者
Xinyi Li,Bingjie Chen,Yaoyao Xie,Yi Luo,Dan Zhu,Lianhui Wang,Shao Su
标识
DOI:10.1021/acs.analchem.4c05329
摘要
The early diagnosis of Alzheimer's disease (AD) plays a vital role in slowing the progression of AD and improving the quality of human life. However, it is still a challenge in the medical field. Herein, an ultrasensitive fluorescent aptasensor was designed for the detection of special phosphorylated tau181 (P-tau181) by coupling with polyvalent aptamers (PAs) structure, hybridization chain reaction (HCR), and the CRISPR-Cas system. Coupling with the signal amplification strategy, the specific recognition ability of the aptamer, and the high cleavage activity of Cas12a protein, the designed aptasensor showed a wide linear range (0.1–106 pg/mL), a low detection limit (0.069 pg/mL), high selectivity, and excellent anti-interference ability for the detection of P-tau181. Moreover, the aptasensor can efficiently analyze P-tau181 in artificial cerebro spinal fluid (aCSF) and serum, proving that it has a promising application in the early diagnosis of AD.
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