拉伤
大肠杆菌
聚对苯二甲酸乙二醇酯
对苯二甲酸
代谢工程
化学
材料科学
酶
生物
生物化学
基因
有机化学
聚酯纤维
复合材料
解剖
作者
Roman M. Dickey,Michaela A. Jones,Neil D. Butler,Ishika Govil,Aditya M. Kunjapur
摘要
Abstract Deconstruction of polyethylene terephthalate (PET) plastic waste generates opportunities for valorization to alternative products. We recently designed an enzymatic cascade that could produce terephthalaldehyde (TPAL) from terephthalic acid. Here, we showed that the addition of TPAL to growing cultures of Escherichia coli wild‐type strain MG1655 and an engineered strain for r educed aromatic a ldehyde re duction (RARE) strain resulted in substantial reduction. We then investigated if we could mitigate this reduction using multiplex automatable genome engineering (MAGE) to create an E. coli strain with 10 additional knockouts in RARE. Encouragingly, we found this newly engineered strain enabled a 2.5‐fold higher retention of TPAL over RARE after 24 h. We applied this new strain for the production of para ‐xylylenediamine (pXYL) and observed a 6.8‐fold increase in pXYL titer compared with RARE. Overall, our study demonstrates the potential of TPAL as a versatile intermediate in microbial biosynthesis of chemicals that derived from waste PET.
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