纤维
化学
生物物理学
离子
水溶液中的金属离子
核心
结晶学
金属
生物化学
有机化学
细胞生物学
生物
作者
Xin Qi,Yulin Li,Mingyue Shen,Qiang Yu,Yi Chen,Jianhua Xie
出处
期刊:Food Chemistry
[Elsevier]
日期:2024-01-01
卷期号:431: 137101-137101
被引量:9
标识
DOI:10.1016/j.foodchem.2023.137101
摘要
The effects of Ca2+, Cu2+, and Fe3+ on rice protein (RP) fibril formation were investigated in this study. Low Ca2+ concentration (≤150 mM) moderately unfolded the conformation of RP, promoting the exposure of hydrophobic sites and RP fibril assembly. Fibril formation was especially promoted with earlier addition of Ca2+. Cu2+ and Fe3+ inhibited RP fibril formation in a dose-dependent manner, and the inhibitory effect of Fe3+ was stronger due to higher affinity with RP. Additionally, the addition of Cu2+ and Fe3+ reduced α-helix and β-sheet contents of RP, respectively, hindering the formation of stacked β-sheet, the main internal structure of fibrils. These two ions also resulted in the formation of random aggregates within 15–50 nm, which further inhibited the conversion of proteins to fibrils. Moreover, Cu2+ and Fe3+ prevented the recruitment of nucleus into fibril-growth-sites, and formed fibrils were disrupted into fragments when these ions were added.
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