Impact of orthophosphate on the light-independent production of reactive oxygen species during microbially-mediated Fe redox cycles

舍瓦内拉 氧化还原 化学 活性氧 过氧化氢 缺氧水域 激进的 氧气 磷酸盐 羟基自由基 无机化学 环境化学 生物化学 细菌 有机化学 生物 遗传学
作者
Nan Xie,Thomas J. DiChristina,Martial Taillefert
出处
期刊:Applied Geochemistry [Elsevier BV]
卷期号:156: 105739-105739
标识
DOI:10.1016/j.apgeochem.2023.105739
摘要

Although light-independent production of reactive oxygen species (ROS) in subsurface environments has been increasingly documented, the processes controlling ROS transformations at redox interfaces remain poorly understood. The Fe(III)-reducing facultative anaerobe Shewanella oneidensis promotes light-independent ROS production in the presence of Fe(III) when alternately exposed to oxic and anoxic conditions and represents a good model of microbial processes at redox interfaces. In this study, S. oneidensis was incubated under three different orthophosphate concentrations from low natural conditions to high growth-promoting conditions to identify the processes regulating the light-independent production of ROS during redox oscillations and determine the impact of this important nutrient on ROS generation. Ferryl complexes were not significant intermediates in the redox cycling of Fe in this system. Instead, hydrogen peroxide (H2O2) and hydroxyl radicals (•OH) represented the main ROS produced in a process that is periodic during redox oscillations. Hydroxyl radicals were mainly produced during the aerated phases in concentrations inversely proportional to orthophosphate concentrations as a result of the complexation of Fe(III) and precipitation of oxidation-resistant vivianite. In relatively low orthophosphate concentration compared to the total reactive sites available on the Fe(III) oxides, •OH was produced at the mineral surface. Increased cell metabolic activities in the highest orthophosphate medium may have increased the •OH production efficiency during the aerated phases by enhancing O2•− formation and/or Fe(III) reduction. Unexpectedly, H2O2 was mainly detected under Fe(III) reduction conditions with net production rates that were proportional to orthophosphate concentrations, except when the solubility of vivianite was exceeded. Hydrogen peroxide is proposed to originate from the reduction of dissolved O2 by Fe(II) produced during dissimilatory Fe(III) reduction in microaerobic conditions. Precipitation of vivianite likely affects the availability of Fe(II) for ROS reactions during redox cycles, thus decreasing overall H2O2 production rates. The rise in metabolic activity, outcompetition of Fe(III) hydrolysis by orthophosphate complexation, and potential dimerization of surface generated •OH are proposed to result in the accumulation of H2O2 under suboxic conditions.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
caibaozi应助风清扬采纳,获得50
刚刚
贪玩小小发布了新的文献求助10
刚刚
汪汪发布了新的文献求助10
1秒前
1秒前
roclie完成签到,获得积分10
1秒前
文静的如娆完成签到,获得积分10
2秒前
3秒前
Yy完成签到,获得积分10
4秒前
5秒前
5秒前
5秒前
dengyan完成签到,获得积分10
6秒前
CodeCraft应助汪汪采纳,获得10
6秒前
我是老大应助刻苦的书竹采纳,获得10
7秒前
智慧爷爷发布了新的文献求助10
7秒前
酷波er应助lw777采纳,获得10
7秒前
岁月如歌发布了新的文献求助10
9秒前
bin完成签到,获得积分10
11秒前
11秒前
很好完成签到,获得积分10
12秒前
小郝已读博完成签到 ,获得积分10
12秒前
12秒前
Self-made完成签到,获得积分10
12秒前
13秒前
14秒前
15秒前
岁月如歌完成签到,获得积分10
16秒前
17秒前
joey完成签到,获得积分10
18秒前
wei完成签到 ,获得积分10
19秒前
miaojuly发布了新的文献求助10
20秒前
20秒前
研友_LXjjOZ发布了新的文献求助150
20秒前
21秒前
22秒前
23秒前
坚定馒头完成签到,获得积分10
23秒前
24秒前
24秒前
星星应助科研通管家采纳,获得30
26秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988868
求助须知:如何正确求助?哪些是违规求助? 3531255
关于积分的说明 11253071
捐赠科研通 3269858
什么是DOI,文献DOI怎么找? 1804822
邀请新用户注册赠送积分活动 881994
科研通“疑难数据库(出版商)”最低求助积分说明 809035