鱼腥草素骨
碱性磷酸酶
骨钙素
WNT3A型
化学
牙髓干细胞
干细胞
MTT法
牙髓(牙)
细胞生长
分子生物学
细胞生物学
牙科
体外
生物
生物化学
医学
Wnt信号通路
基因
酶
作者
Yanyan Sun,Mengtong Yuan,Xin Shi,Mingyue Liu,Weiping Hu
出处
期刊:PubMed
日期:2016-10-09
卷期号:51 (10): 634-639
标识
DOI:10.3760/cma.j.issn.1002-0098.2016.10.012
摘要
Objective: To investigate the effects of Wnt3a protein on proliferation and osteogenic differentiation of human dental pulp stem cells(DPSC). Methods: Intact human permanent teeth extracted for orthodontic reasons were collected and used as study models. The biological effects of Wnt3a on DPSC were investigated using methyl thiazolyl tetrazolium(MTT), alkaline phosphatase(ALP) activity assay, alizarin red S staining and realtime fluorescence quantitative PCR. Osteogenic-related gene expression of induced DPSC was examinedby using tests of bone sialoprotein(BSP), osteocalcin(OCN), collagen type Ⅰ (COL-Ⅰ) and Runt-related transcription factor 2(RUNX-2). Results: Wnt3a proteininduced an increase of cell growth and treatment of DPSC with Wnt3a induced a highest increase in cell growth at the concentration of 5 μg/L. 5 μg/L Wnt3a proteins combined with the osteogenic medium treatment caused up-regulated osteogenic differentiation, ALP activity and express of osteogenic-related genes of DPSC, and the ALP activity(0.47±0.04) was significantly stronger than the other groups(osteogenic medium: 0.39±0.05; 20 μg/L: 0.34±0.03; 50 μg/L: 0.27±0.07; 100 μg/L: 0.20±0.03). Conclusions: Exogenous Wnt3a protein treatment on DPSC could affect the proliferation and osteogenic differentiation.
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