初乳
免疫分析
检出限
生物传感器
哺乳期
化学
色谱法
食品科学
表面等离子共振
牛血清白蛋白
脱脂牛奶
多克隆抗体
动物科学
抗体
生物
生物化学
材料科学
免疫学
纳米技术
纳米颗粒
遗传学
怀孕
作者
Harvey E. Indyk,Selena Hart,Theo Meerkerk,Brendon D. Gill,David C Woollard
标识
DOI:10.1016/j.idairyj.2017.05.010
摘要
An optical biosensor immunoassay exploiting surface plasmon resonance is described for the quantification of β-lactoglobulin in milk. Samples were diluted with buffer, and the protein estimated from binding with a polyclonal antibody immobilised on the sensor surface. Analytical method performance characteristics including range, detection limit, precision and accuracy were determined and reported. The temporal variability in the β-lactoglobulin content of milk from pasture-fed cows during early lactation and across a production season was investigated. The content of β-lactoglobulin decreased from >10 mg mL−1 in early colostrum to <5 mg mL−1 in mature milk, and the β-lactoglobulin content of skim milk powder trended from 25 to 60 mg g−1 across a season. In view of its allergenicity, these data will improve understanding of the expression of innate β-lactoglobulin in the milk of pasture-grazed dairy herds, thereby providing information that is applicable to the formulation of bovine milk-based products.
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