Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumberApostichopus japonicus

Aquaculture ResearchVolume 48, Issue 12 p. 5883-5891 ORIGINAL ARTICLE Carbohydrate and amino acids metabolic response to heat stress in the intestine of the sea cucumber Apostichopus japonicus Dongxue Xu, Corresponding Author Dongxue Xu dongxuexu1989@126.com orcid.org/0000-0002-5560-3306 College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China Correspondence Dr. Dongxue Xu, College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China. Email: dongxuexu1989@126.comSearch for more papers by this authorShun Zhou, Shun Zhou College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, ChinaSearch for more papers by this authorHongsheng Yang, Hongsheng Yang Key laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaSearch for more papers by this author Dongxue Xu, Corresponding Author Dongxue Xu dongxuexu1989@126.com orcid.org/0000-0002-5560-3306 College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China Correspondence Dr. Dongxue Xu, College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, China. Email: dongxuexu1989@126.comSearch for more papers by this authorShun Zhou, Shun Zhou College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, ChinaSearch for more papers by this authorHongsheng Yang, Hongsheng Yang Key laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, ChinaSearch for more papers by this author First published: 02 June 2017 https://doi.org/10.1111/are.13411Citations: 19Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract The sea cucumber Apostichopus japonicus is an economic species mainly distributed along the coast of northern China, south-eastern Russia, Japan, Republic of Korea and Democratic People's Republic of Korea. Aquaculture industry of A. japonicus has been facing severe challenge of high temperature. In this study, we studied the mRNA expression profiles of eight key metabolic enzymes involved in carbohydrate and amino acids metabolism in A. japonicus under heat stress. The expression of hexokinase, pyruvate kinase and malate dehydrogenase showed downregulated response to heat stress, while the expression of glutamate dehydrogenase, alanine transaminase and branched-chain aminotransferase showed upregulated response. In addition, the expression of lactate dehydrogenase and glutamate synthase showed no significant difference. We also applied 1H NMR-based metabolomics to investigate metabolic changes in the intestine tissue of A. japonicus under heat stress, the results of which revealed nine increased and 10 decreased metabolites in the heat stress group. These response genes and metabolites have potential to become markers for identifying severity of heat stress. More importantly, our findings suggest significant links between gene expression and metabolites changing, highlighting regulation networks of carbohydrate and amino acid metabolism in heat-stressed A. japonicus. Citing Literature Volume48, Issue12December 2017Pages 5883-5891 RelatedInformation