已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Schisandrin C attenuates renal damage in diabetic nephropathy by regulating macrophage polarization

白细胞介素 糖尿病肾病 肿瘤坏死因子α 巨噬细胞极化 药理学 STAT蛋白 化学 医学 车站3 内分泌学 细胞凋亡 内科学 细胞因子 巨噬细胞 体外 生物化学
作者
Yu Wang,Jingqiu Cui,Ming Liu,Yingqi Shao,Xiaoying Dong
出处
期刊:American Journal of Translational Research [e-Century Publishing Corporation]
卷期号:13 (1): 210-222 被引量:12
链接
标识
摘要

This study aimed to investigate the protective effects of Schisandrin C during diabetic nephropathy (DN) treatment. After DN induction, mice were treated with Schisandrin C, and diabetic metabolic parameters and renal function-associated factors were measured. Renal structural damage was evaluated by hematoxylin and eosin (HE) and Masson's trichrome staining. Macrophage polarization and macrophage-mediated inflammatory factors were detected in the kidneys by immunohistochemistry (IHC) and enzyme-linked immunosorbent assay (ELISA), respectively. The Swiprosin-1/interferon (IFN)-γ-Rβ pathway was evaluated by western blot (WB) analysis. The preliminary effects of Schisandrin C in high-glucose-stimulated macrophages from DN mice were verified by flow cytometry, ELISA, and WB analyses. These results indicated that Schisandrin C significantly regulated physiological parameters in DN. Renal structural damage was mitigated by Schisandrin C. In Schisandrin-C-treated groups, the expression levels of CD86, tumor necrosis factor (TNF)-α, interleukin (IL)-6, and IL-1β decreased, whereas CD206, IL-10, and transforming growth factor (TGF)-β expression levels increased. In vitro experiments indicated that among CD86+ cells, TNF-α, IL-6, and IL-1β expression levels significantly decreased, whereas among CD206+ cells, IL-10 and TGF-β expression increased following Schisandrin-C-treatment. Finally, Schisandrin C inhibited the expression of Swiprosin-1, IFN-γ-Rβ, phospho-Janus kinase 2 (p-JAK2), phospho-signal transducer and activator of transcription 1 (p-STAT1), and p-STAT3, in both DN model mice and high-glucose-stimulated RAW264.7 cells. The present study indicated a novel use for Schisandrin C to suppress DN progression, by promoting M1 to M2 macrophage polarization. Schisandrin C exerted protective effects against DN by regulating the polarization-dependent Swiprosin-1/IFN-γ-Rβ signaling pathway in macrophages.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
3秒前
3秒前
田様应助科研通管家采纳,获得10
3秒前
猪猪hero应助科研通管家采纳,获得10
3秒前
领导范儿应助科研通管家采纳,获得10
3秒前
wanci应助科研通管家采纳,获得10
3秒前
在水一方应助科研通管家采纳,获得10
3秒前
我是老大应助科研通管家采纳,获得10
3秒前
3秒前
猪猪hero应助科研通管家采纳,获得10
4秒前
Venovenom发布了新的文献求助30
4秒前
情怀应助tt采纳,获得10
5秒前
Akim应助坚强枫采纳,获得10
6秒前
6秒前
9秒前
鼠鼠完成签到 ,获得积分10
11秒前
onlyan发布了新的文献求助20
12秒前
mpenny77发布了新的文献求助30
12秒前
十四发布了新的文献求助10
13秒前
15秒前
脑洞疼应助yaling采纳,获得10
18秒前
18秒前
19秒前
mpenny77完成签到,获得积分10
21秒前
多肉葡萄完成签到 ,获得积分10
23秒前
SciGPT应助大面包采纳,获得10
24秒前
25秒前
cc发布了新的文献求助10
25秒前
Rondab应助十四采纳,获得10
29秒前
怕孤独的访云完成签到 ,获得积分10
29秒前
SYLH应助晶晶采纳,获得10
31秒前
kokoko完成签到,获得积分10
31秒前
31秒前
夙夙发布了新的文献求助10
33秒前
33秒前
大面包发布了新的文献求助10
36秒前
38秒前
Sophia发布了新的文献求助10
39秒前
大头完成签到,获得积分0
42秒前
onlyan完成签到,获得积分10
43秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989857
求助须知:如何正确求助?哪些是违规求助? 3531994
关于积分的说明 11255679
捐赠科研通 3270758
什么是DOI,文献DOI怎么找? 1805053
邀请新用户注册赠送积分活动 882195
科研通“疑难数据库(出版商)”最低求助积分说明 809208