Programmed cell death process in freshwater Microcystis aeruginosa and marine Phaeocystis globosa induced by a plant derived allelochemical

Harmful algal blooms (HAB) are a serious problem worldwide. Allelochemicals from natural plants were recently thought to be promising anti-algaecide in controlling harmful algae. However, the programmed cell death (PCD) process of algae under allelopathic pressure induced by 5,4'-dihydroxyflavone (5,4'-DHF) was poorly understood. In this study, two common and worldwide distributed microalgae, Microcystis aeruginosa and Phaeocystis globosa were selected as target algae, and the PCD processes induced by 5,4'-DHF were cross-compared between the two species. Both algae species were inhibited significantly by 5,4'-DHF with the relative sensitivity of 0.11. To uncover the PCD progress systematically, signals for PCD triggering, antioxidant enzyme activity, photosynthetic ability variation, caspase-like activities and typical indicators were investigated. In both species, typical indicators of PCD - phosphatidylserine externalization and chromatin condensation - were detected. The intracellular reactive oxygen species (ROS), nitric oxide (NO) and H2O2 were the potential signal molecules to stimulate PCD, and caspase-like activities were activated with an elevation of cytochrome c indicating the initiation of PCD in both species. However, P. globosa responded to 5,4'-DHF immediately after 3 h with the elevation of ROS and not in M. aeruginosa. Antioxidant enzyme activities of superoxide dismutase (SOD) and catalase (CAT) in M. aeruginosa and P. globosa also showed different patterns on day 3. Specifically, SOD activity in M. aeruginosa increased significantly while it decreased significantly in P. globosa, CAT activity in M. aeruginosa decreased significantly while it increased significantly in P. globosa (p < 0.05). Malondialdehyde (MDA) content in P. globosa increased significantly (p < 0.001) while it showed no variation in M. aeruginosa. Overall, this study is one of the earliest studies to explore the inhibition and action mechanism of plant derived flavonoids on harmful algae from the perspective of PCD, and provide new insights into the antialgal mechanism of allelochemicals.