生物
中央凹
视网膜
视网膜
中央凹
诱导多能干细胞
视觉光转导
神经科学
色素性视网膜炎
解剖
作者
Aindrila Saha,Elizabeth E. Capowski,Maria A Fernandez Zepeda,Emma C Nelson,David M. Gamm,Raunak Sinha
标识
DOI:10.1016/j.stem.2022.01.002
摘要
High-definition vision in humans and nonhuman primates is initiated by cone photoreceptors located within a specialized region of the retina called the fovea. Foveal cone death is the ultimate cause of central blindness in numerous retinal dystrophies, including macular degenerative diseases. 3D retinal organoids (ROs) derived from human pluripotent stem cells (hPSCs) hold tremendous promise to model and treat such diseases. To achieve this goal, RO cones should elicit robust and intrinsic light-evoked electrical responses (i.e., phototransduction) akin to adult foveal cones, which has not yet been demonstrated. Here, we show strong, graded, repetitive, and wavelength-specific light-evoked responses from RO cones. The photoresponses and membrane physiology of a significant fraction of these lab-generated cones are comparable with those of intact ex vivo primate fovea. These results greatly increase confidence in ROs as potential sources of functional human cones for cell replacement therapies, drug testing, and in vitro models of retinal dystrophies.
科研通智能强力驱动
Strongly Powered by AbleSci AI