流式细胞术
免疫系统
生物
抗体
免疫
表型
计算生物学
免疫学
遗传学
基因
作者
Nadine M. Weisel,Stephen Joachim,Shuchi Smita,Derrick Callahan,Rebecca A. Elsner,Laura J. Conter,Maria Chikina,Donna L. Farber,Florian Weisel,Mark J. Shlomchik
标识
DOI:10.1038/s41590-021-01078-x
摘要
Memory B cells (MBCs) protect the body from recurring infections. MBCs differ from their naive counterparts (NBCs) in many ways, but functional and surface marker differences are poorly characterized. In addition, although mice are the prevalent model for human immunology, information is limited concerning the nature of homology in B cell compartments. To address this, we undertook an unbiased, large-scale screening of both human and mouse MBCs for their differential expression of surface markers. By correlating the expression of such markers with extensive panels of known markers in high-dimensional flow cytometry, we comprehensively identified numerous surface proteins that are differentially expressed between MBCs and NBCs. The combination of these markers allows for the identification of MBCs in humans and mice and provides insight into their functional differences. These results will greatly enhance understanding of humoral immunity and can be used to improve immune monitoring.
科研通智能强力驱动
Strongly Powered by AbleSci AI