幽门螺杆菌
抗体
胃
蛋黄
微生物学
生物
免疫系统
螺旋藻科
免疫球蛋白G
胃炎
免疫学
生物化学
食品科学
遗传学
作者
Kyung Sook Hong,Mi‐Ran Ki,H. M. Arif Ullah,Eun‐Joo Lee,Yong Deuk Kim,Myung-Jin Chung,Ahmed K. Elfadl,Jin‐Kyu Park,Kyu‐Shik Jeong
出处
期刊:Vaccine
[Elsevier]
日期:2018-01-01
卷期号:36 (3): 371-380
被引量:25
标识
DOI:10.1016/j.vaccine.2017.11.082
摘要
Helicobacter pylori, a gram-negative bacterium, is the causative agent of gastric disorders and gastric cancer in the human stomach. Vacuolating cytotoxin A (VacA) is among the multi-effect protein toxins released by H. pylori that enables its persistence in the human stomach. To evaluate the effect of anti-VacA egg yolk immunoglobulin (anti-VacA IgY) on H. pylori infection, a highly specific anti-VacA IgY was produced from egg yolks of hens immunized with a mixture of two purified recombinant VacAs. Female C57BL/6 mice were supplemented anti-VacA IgY daily with drinking water for 2 weeks before and 4 weeks after H. pylori ATCC 43504 inoculation. Anti-VacA IgY recognized both native and denatured structures of VacA by enzyme-linked immunosorbent assay and immunoblotting analyses, respectively. Oral administration of anti-VacA IgYs significantly (p < .05) reduced the serum levels of anti-H. pylori antibodies compared to those in the H. pylori-infected, untreated group. The reduction in the immune response was accompanied by a significant (p < .05) decrease in eosinophilic infiltration of the stomach in anti-VacA IgY treated group compared to other groups. Concomitantly, H. pylori–induced histological changes and H. pylori antigen-positivity in gastric tissues were decreased significantly (p < .05) in anti-VacA IgY treated group similar to the control group. Oral administration of anti-VacA IgY is correlated with a protective effect against H. pylori colonization and induced histological changes in gastric tissues. Our experimental study has proved that it is expected to be a new drug candidate of Hp infection by further study.
科研通智能强力驱动
Strongly Powered by AbleSci AI