Melanogenesis Inhibitory and Antioxidant Effects of Camellia oleifera Seed Oil

Purpose: The study aimed to characterize the fatty acid profile of Camellia oleifera (tea) seed oil and evaluate for cytotoxicity and activities on melanogenesis and antioxidant activity assays in order to utilize as the functional oil. Methods: The fatty acid profile of oil was analyzed by gas chromatography/mass spectrometry (GC/MS). The cytotoxicity was performed by sulforhodamine B (SRB) assay in B16-F10 melanoma cells and 3T3-L1 cells. The melanogenesis assay, including melanin content and activities of tyrosinase and tyrosinase-related protein-2 (TRP-2), and antioxidant activity were evaluated. Results: Three major fatty acids of oil were oleic acid (87.93±0.19%), stearic (5.14±0.06%) and palmitic (5.08±0.12%) acids. The non-cytotoxicity of 5% tea seed oil demonstrated the cell viabilities of 94.59±3.41% in B16-F10 melanoma cells and 97.57±1.62% in 3T3-L1 cells. Tea seed oil exhibited the inhibitory activity on melanogenesis assay via inhibition of tyrosinase and TRP-2 activities. The antioxidant activity of 3% tea seed oil appeared the cellular protection with cell viability of 90.38±7.77%. Conclusion: The results of study have shown the potential utilization of tea seed oil as the functional oil in several products, including health, food and cosmetic products.