In this study, MoS2 nanosheets were prepared using a facile hydrothermal method and we studied their electromagnetic wave shielding performance for the first time. The results showed that the nanosheets synthesized with a reaction time of 10 h exhibited excellent electromagnetic shielding performance and the attenuation of electromagnetic waves was greater than 20 dB in the frequency range from 2 to 18 GHz. These findings may facilitate the development of novel layered nanomaterials with excellent electromagnetic shielding properties.