基因
外周血单个核细胞
生物
基因表达
微阵列
分子生物学
遗传学
免疫学
体外
作者
Yingren Zhao,Yingli He,Shulin Zhang,Yuan Yang,Min Liu,Yan Jin,Jinfeng Liu
出处
期刊:PubMed
日期:2005-11-01
卷期号:13 (11): 811-4
摘要
To investigate the peripheral blood monocyte (PBMC) gene expression profile in a familial clustering of patients with chronic hepatitis B (CHB).cRNA prepared from PBMC in a family with 5 CHB patients and 4 normal controls was hybridized to high-density oligouncleotide arrays (HG-U133A 2.0 Human GeneChips, Affymetrix), which interrogate the expression of approximately 22,000 human ESTs. Primary image obtained from scanning was analysed with a DNT software package. Real-time PCR was employed to confirm the gene chip results.55 genes out of 22,000 ESTs were identified differently. Among the 55 genes 14 showed increased expression and 41 showed decreased expression in the familial clustering CHB patients compared with those in normal controls. Most of the genes (57%) were involved in immunity, inflammation, apoptosis, signaling transduction, and cell cycle.These results suggest that the hosts with this broad range of gene expression alterations are susceptible to hepatic B infection.
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