过氧亚硝酸盐
化学
活性氧
体内
炎症
内生
荧光
体外
生物化学
细胞生物学
生物物理学
免疫学
超氧化物
生物
酶
生物技术
物理
量子力学
作者
Yuxiang Ji,Lei Sha,Jian Zhang,L. J. Qu,Jinsheng Wu,Heng Liu,Ziyi Cheng
标识
DOI:10.1016/j.bioorg.2023.106650
摘要
As one of the important members of reactive oxygen species, ONOO− plays a crucial role in signal transduction, immune response, and other physiological activities. Aberrant changes in ONOO− levels in the living organism are usually associated with many diseases. Therefore, it is important to establish a highly selective and sensitive method for the determination of ONOO− in vivo. Herein, we designed a novel ratio near-infrared fluorescent probe for ONOO− by directly conjugating dicyanoisophorone (DCI) to hydroxyphenyl-quinazolinone (HPQ). Surprisingly, HPQD was unaffected by environmental viscosity and responded rapidly to ONOO− within 40 s. The linear range of ONOO− detection was from 0 μM to 35 μM. Impressively, HPQD did not react with reactive oxygen species and was sensitive to exogenous/endogenous ONOO− in live cells. We also investigated the relationship between ONOO− and ferroptosis and achieved in vivo diagnosis and efficacy evaluation of mice model of LPS-induced inflammation, which showed promising prospects of HPQD in ONOO−-related studies.
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