生物
黄烷酮
黄酮醇
柚皮素
经络
基因家族
基因
类黄酮生物合成
花青素
类黄酮
双加氧酶
查尔酮合酶
生物化学
拟南芥
生物合成
异源表达
植物
基因表达
转录组
突变体
抗氧化剂
重组DNA
作者
Samiksha Saxena,Gaurav Pal,Ashutosh Pandey
出处
期刊:Plant Science
[Elsevier]
日期:2023-11-01
卷期号:336: 111836-111836
被引量:4
标识
DOI:10.1016/j.plantsci.2023.111836
摘要
Chickpea is an important leguminous crop plant with two cultivated types, desi and kabuli. It is nutritionally enriched in flavonoid content in addition to minerals and vitamins imparting huge health benefits to human beings. Our study elucidates the functionality of 2-oxoglutarate dependent dioxygenase (2-ODD) gene family members i.e., flavanone-3-hydroxylase (F3H), flavonol synthase (FLS) and anthocyanidin synthase (ANS) in chickpea using heterologous bacterial system and in-planta studies in Arabidopsis. This provides information about the biosynthesis of two very significant sub-classes of flavonoids- flavonols and anthocyanins. Here, we show that all the three homologs of F3H in chickpea can utilize not just naringenin but also eriodictyol as their substrate. Moreover, we show that FLS in chickpea exhibits bifunctionality having both FLS and F3H activity. Also, our study indicates the richness of desi chickpea over kabuli type through gene expression and metabolite content analyses. Overall, our study establishes the functionality of 2-ODD gene family involved in the early and late steps of flavonoid biosynthesis pathway in chickpea. It paves way for better genetic manipulation of the pathway for direct or indirect synthesis of three major subclasses of flavonoids (flavonol, anthocyanin and proanthocyanin) to develop nutritious, environmentally stable and healthy chickpea (Cicer arietinum) crop.
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