Isolation and characterization of quercetin from Prunus Serrulata leaf extract in the synthesis of Fe3O4 nanoparticles

Flavonoids and their derivatives have been known as reducing and capping agents for the synthesis of nanoparticles. These flavonoids are effective in the green synthesis of nanoparticles due to their environmentally friendly nature, low cost, and energy and time-saving assets. Generally, it is ignored which type of flavonoids were utilized in the synthesis of nanoparticles. This study presents the isolation and characterization of quercetin from Prunus Serrulata (P. Serrulata) leaf extract for the first time as the responsible reducing and capping agent in the synthesis of iron oxide nanoparticles (Fe3O4 NPs). The morphological characterization of the nanoparticles was performed by utilizing Scanning Electron Microscopy (SEM), Scanning Transmission Electron Microscopy (STEM), and Energy Dispersive X-ray Spectroscopy (EDX) analyses, whereas flavonoid characterization was completed through Ultraviolet–Visible Spectroscopy (UV–Vis), Fourier Transform-Infrared Spectroscopy (FT-IR), Liquid Chromatography-Mass Spectroscopy (LC-MS), and Nuclear Magnetic Resonance Spectroscopy (NMR). By using optimized conditions as 3:1 (Fe+3 solution: Prunus serrulata leaf extract) mixing ratio, 10 mM Fe+3 precursor, pH 6, and 25 °C reaction temperature, the average particle sizes of synthesized Fe3O4 NPs were ∼ 40 nm with the spherical shape. R2. Some quantitative results need to be added in the abstract.