噬菌体展示
计算生物学
DNA测序
深度测序
选择(遗传算法)
工作流程
生物
基因组文库
遗传学
计算机科学
肽序列
DNA
基因
基因组
人工智能
数据库
抗体
作者
Vincent Van Deuren,Sander Plessers,Rob Lavigne,Johan Robben
出处
期刊:Methods in molecular biology
日期:2023-11-16
卷期号:: 333-345
被引量:1
标识
DOI:10.1007/978-1-0716-3549-0_20
摘要
This chapter describes the workflow to implement deep sequencing into standard phage display experiments on protein libraries. By harvesting the power of high throughput of these techniques, it allows for comprehensive analysis of the naïve library and library evolution in response to selection by ligand binding. The mutagenized target region of the protein variants encoded by the phage pool is analyzed by Illumina paired-end sequencing. Sequence data are processed to extract selection-enriched amino acid motifs. In addition, a complementary long-read sequencing approach is proposed enabling the monitoring of display vector stability.
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