Cryptosporidium equi n. sp. (Apicomplexa: Cryptosporidiidae): Biological and genetic characterisations

生物 隐孢子虫 基因型 微小隐孢子虫 顶复亚门 系统发育树 基因 微生物学 病毒学 遗传学 粪便 恶性疟原虫 免疫学 疟疾
作者
Jianbo Huang,Ming Chen,Yongli He,Haoyu Chen,Mingming Huang,Na Li,Una Ryan,Martin Kváč,Yaoyu Feng,Lihua Xiao,Yaqiong Guo
出处
期刊:International Journal for Parasitology [Elsevier BV]
卷期号:53 (10): 545-554 被引量:19
标识
DOI:10.1016/j.ijpara.2023.02.008
摘要

The horse genotype is one of three common Cryptosporidium spp. in equine animals and has been identified in some human cases. The species status of Cryptosporidium horse genotype remains unclear due to the lack of extensive morphological, biological, and genetic data. In the present study, we have conducted biological and whole genome sequence analyses of an isolate of the genotype from hedgehogs and proposed to name it Cryptosporidium equi n. sp. to reflect its common occurrence in equine animals. Oocysts of C. equi measured 5.12 ± 0.36 μm × 4.46 ± 0.21 μm with a shape index of 1.15 ± 0.08 (n = 50). Cryptosporidium equi was infectious to 3-week-old four-toed hedgehogs (Atelerix albiventris) and mice, with a prepatent period of 2-9 days and a patent period of 30-40 days in hedgehogs. It was not infectious to rats and rabbits. Phylogenetic analyses of small subunit rRNA, 70 kDa heat shock protein, actin, 60 kDa glycoprotein and 100 other orthologous genes revealed that C. equi is genetically distinct from other known Cryptosporidium species and genotypes. The sequence identity between C. equi and Cryptosporidium parvum genomes is 97.9%. Compared with C. parvum, C. equi has lost two MEDLE genes and one insulinase-like protease gene and gained one SKSR gene. In addition, 60 genes have highly divergent sequences (sequence differences ≥ 5.0%), including those encoding mucin-like glycoproteins, insulinase-like peptidases, and MEDLE and SKSR proteins. The genetic uniqueness of C. equi supports its increasing host range and the naming of it as a valid Cryptosporidium species. This is the first known use of whole genome sequence data in delineating new Cryptosporidium species.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小曾应助pakiorder采纳,获得10
3秒前
wxy完成签到,获得积分10
4秒前
分析完成签到 ,获得积分10
4秒前
hulin_zjxu完成签到,获得积分10
4秒前
桃紫完成签到,获得积分10
6秒前
小董不懂完成签到,获得积分10
7秒前
椰子完成签到,获得积分10
7秒前
沐沐溪三清完成签到,获得积分10
7秒前
8秒前
刘丰完成签到 ,获得积分10
10秒前
郑桂庆完成签到 ,获得积分10
11秒前
zhang完成签到 ,获得积分10
11秒前
yuchen完成签到,获得积分10
12秒前
喜悦的水云完成签到 ,获得积分10
12秒前
13秒前
zhaokunfeng完成签到,获得积分10
13秒前
Y123发布了新的文献求助10
13秒前
wu完成签到,获得积分10
13秒前
高高诗柳完成签到 ,获得积分10
13秒前
王金豪完成签到,获得积分10
13秒前
LSS完成签到,获得积分10
13秒前
榜一大哥的负担完成签到 ,获得积分10
14秒前
Lucas应助qi0625采纳,获得10
14秒前
顾矜应助以筱采纳,获得10
15秒前
景清完成签到,获得积分10
15秒前
细心香烟完成签到 ,获得积分10
15秒前
hu完成签到 ,获得积分10
15秒前
HQ完成签到,获得积分10
15秒前
16秒前
水清木华完成签到,获得积分10
16秒前
17秒前
miao完成签到,获得积分20
17秒前
xyp_zjut应助学术乞丐采纳,获得10
17秒前
Lucas应助凉白开采纳,获得10
17秒前
体贴凌柏发布了新的文献求助10
18秒前
18秒前
18秒前
鹿子完成签到 ,获得积分10
18秒前
秋枫忆完成签到,获得积分10
20秒前
宋立发布了新的文献求助10
20秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038368
求助须知:如何正确求助?哪些是违规求助? 3576068
关于积分的说明 11374313
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029