Activation of the inflammasome and pyroptosis cascade in podocytes of patients with minimal change disease

上睑下垂 炎症体 级联 微小变化病 疾病 细胞生物学 化学 医学 癌症研究 神经科学 免疫学 生物 炎症 内科学 肾小球肾炎 局灶节段性肾小球硬化 色谱法
作者
Yuki Kajio,Taihei Suzuki,Kazuki Kobayashi,Nobuhiro Kanazawa,Masayuki Iyoda,Hirokazu Honda,Kazuho Honda
出处
期刊:Ndt Plus [Oxford University Press]
卷期号:17 (8)
标识
DOI:10.1093/ckj/sfae216
摘要

ABSTRACT Background In contrast to childhood minimal change disease (MCD), adult-onset MCD frequently recurs and requires prolonged immunosuppressive therapy. Accordingly, an investigation of the pathogenesis of adult MCD is required. MCD is usually accompanied by severe dyslipidaemia. Oxidized low-density lipoprotein (ox-LDL) is known to function in a damage-associated molecular pattern (DAMP) through CD36, triggering the NOD-like receptor thermal protein domain-associated protein 3 (NLRP3) inflammasome and programmed cell death called pyroptosis. However, the relationship between MCD pathogenesis and NLRP3 inflammasome/pyroptosis activation via CD36 is not fully understood. Methods We conducted comprehensive histological and clinical evaluations by analysing renal biopsy (RBx) specimens and urine samples obtained from 26 patients with MCD. These samples were compared with control kidneys from 15 transplant donors and urine samples from 15 healthy volunteers. Results The number of podocytes was lower in the MCD group than in the control group. Urinary ox-LDL levels were higher in the MCD group than in the control group. Immunofluorescence staining revealed that NLRP3 and CD36 were upregulated in MCD podocytes. Urinary interleukin (IL)-18 levels increased in patients with MCD. Steroid therapy performed before RBx appeared to maintain the podocyte number and reduce urinary ox-LDL and IL-18 levels. Conclusion In MCD, the NLRP3 inflammasome and pyroptosis cascade seem to be activated via upregulation of CD36 in podocytes, associated with increased urinary ox-LDL. Elevated urinary IL-18 levels suggest that pyroptosis may occur in MCD. Further research is required to confirm the significance of the podocyte NLRP3 inflammasome/pyroptosis in MCD.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
亮山火马完成签到,获得积分10
1秒前
旺旺碎冰冰完成签到,获得积分10
1秒前
zhong完成签到,获得积分10
1秒前
厚厚厚厚厚丶完成签到,获得积分10
1秒前
2秒前
拼搏的青雪完成签到,获得积分10
2秒前
liuliu完成签到 ,获得积分10
3秒前
3秒前
汉中太守魏延完成签到,获得积分10
3秒前
siyan156完成签到,获得积分10
4秒前
ZeradesY完成签到,获得积分10
4秒前
牛轧糖完成签到 ,获得积分10
4秒前
吴帆完成签到,获得积分10
6秒前
米粥饭完成签到,获得积分10
7秒前
223311完成签到,获得积分10
7秒前
WENBO完成签到,获得积分10
8秒前
8秒前
shamy夫妇完成签到,获得积分10
9秒前
chcmuer完成签到,获得积分10
11秒前
曾经的听云完成签到 ,获得积分10
11秒前
sapphire_yy完成签到,获得积分10
11秒前
鹿谷波发布了新的文献求助10
11秒前
aaaa完成签到,获得积分10
12秒前
guanzhuang完成签到,获得积分10
12秒前
搞不动科研完成签到,获得积分10
13秒前
LaFee完成签到,获得积分10
13秒前
15秒前
不回首完成签到 ,获得积分10
15秒前
15秒前
yzlsci完成签到,获得积分0
15秒前
美满的泥猴桃完成签到,获得积分10
16秒前
儒雅的焦完成签到,获得积分10
16秒前
16秒前
amber发布了新的文献求助10
17秒前
跳跳妈妈完成签到,获得积分10
17秒前
从容的幻柏完成签到,获得积分10
17秒前
17秒前
LXL完成签到,获得积分10
18秒前
Johnlian完成签到 ,获得积分10
18秒前
桐桐应助Arthur采纳,获得10
18秒前
高分求助中
Sustainability in Tides Chemistry 2000
Microlepidoptera Palaearctica, Volumes 1 and 3 - 13 (12-Volume Set) [German] 1122
Дружба 友好报 (1957-1958) 1000
The Data Economy: Tools and Applications 1000
Mantiden - Faszinierende Lauerjäger – Buch gebraucht kaufen 700
PraxisRatgeber Mantiden., faszinierende Lauerjäger. – Buch gebraucht kaufe 700
A Dissection Guide & Atlas to the Rabbit 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3099877
求助须知:如何正确求助?哪些是违规求助? 2751333
关于积分的说明 7612942
捐赠科研通 2403282
什么是DOI,文献DOI怎么找? 1275217
科研通“疑难数据库(出版商)”最低求助积分说明 616310
版权声明 599053