电化学发光
纳米团簇
生物传感器
氢氧化物
胶质纤维酸性蛋白
纳米技术
材料科学
化学
检出限
无机化学
色谱法
生物
免疫组织化学
免疫学
作者
Lin-Lei Liu,Xiang Lian,Yaqin Chai,Ruo Yuan
标识
DOI:10.1016/j.bios.2024.116685
摘要
In this work, the copper nanoclusters (Cu NCs) were confined on 3D layered double hydroxide (3D-LDH) to form Cu NCs@3D-LDH with outstanding electrochemiluminescence (ECL) for constructing ultrasensitive biosensor to detect of glial fibrillary acidic protein (GFAP) implicated in Alzheimer's Disease (AD). More importantly, compared to the individual Cu NCs, Cu NCs@3D-LDH presented strong and stable ECL response, since 3D-LDH could not only gather more Cu NCs but also limit the intramolecular free motion to reduce nonradiative transition for obtaining high ECL intensity. In addition, the improved cascade amplification method combining proximity ligation assay (PLA) with DNAzyme could transform tiny amount of target protein into a large amount of output DNA to improve sensitivity of biosensor. The ECL biosensor realized ultrasensitive detection of GFAP with the detection limit of 2 ag/mL and it had been successfully applied to the evaluation of GFAP in the serum of patients with neurological diseases. This research offered a general and facile method to improve ECL performance of Cu NCs for sensitive detection of biomarkers for disease diagnosis.
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