Endogenously Activated and Self‐Reinforced DNA Lipid Nanodevice for Spatial‐Specific and High‐Contrast Imaging of MicroRNA in Cells and Animals

纳米器件 材料科学 小RNA 体内 DNA 纳米技术 内生 生物物理学 细胞生物学 生物 生物化学 基因 生物技术
作者
Chan Li,Haiyan Jia,Wangxin Zhao,Rong Wu,Xiaoyu Zhou,Xiaoling Wei,Guohui Xue,Zhifa Shen,Chang Xue
出处
期刊:Advanced Functional Materials [Wiley]
卷期号:34 (40) 被引量:3
标识
DOI:10.1002/adfm.202405780
摘要

Abstract Tumor‐specific microRNA (miRNA) imaging strategies are critical for investigating mechanisms associated with cancer progression; however, nonspecific signal leakage and false‐positive signaling limit their selectivity and efficiency. In this paper, an endogenously activated and self‐reinforced DNA lipid nanodevice (LND) for spatial‐specific and high‐contrast imaging of miRNAs in living cells and animals is presented. The LND is functionalized on cholesterol‐based lipid micelles (CLMs) containing smart response and self‐fueling DNA components that display redox‐activatable and autocatalytic miRNA probing activities, respectively. The LNDs are initially silenced and selectively activated using glutathione, an endogenous microenvironmental factor overexpressed in tumor cells, to prevent nonspecific signal leakage. Subsequently, an autocatalytic DNA circuit is used for tumor‐specific and high‐contrast miRNA imaging. It is demonstrated that robust CLM nanoparticles can be easily assembled from a cholesterol‐conjugated G‐rich DNA sequence by stirring in a buffered solution, enabling the LND to enter cells naturally. In addition, in vitro and in vivo data illustrate that the enhanced biostability of DNA components on the surface of LNDs can be realized for minimizing false‐positive signals, making the system ideal for miRNA imaging in living mice. This smart LND technique has great potential for precise biomedical imaging and clinical diagnosis.
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