低温保存
扩展器
精子
氧化应激
线粒体
脂质过氧化
精子活力
男科
精液冷冻保存
超氧化物
化学
活性氧
生物
生物化学
细胞生物学
医学
酶
胚胎
有机化学
聚氨酯
作者
Arjun Venkateshappa,Pradeep Kumar,Ravi Dutt,Amit Kumar,Renu Bala,Nisha Verma,A. Jérome,Meenakshi Virmani,C.S. Patil,Shivani Bhardwaj,Dharmendra Kumar,Prem Singh Yadav
出处
期刊:Andrologia
[Wiley]
日期:2022-04-22
卷期号:54 (7)
被引量:9
摘要
Sperm mitochondrion is one of the major susceptible organelles that get damaged during cryopreservation. The study aimed to minimize mitochondrial dysfunction and oxidative stress during sperm cryopreservation using mitochondria-specific antioxidants. For this, semen was collected from five buffalo bulls (3 ejaculates/bull). The ejaculates were diluted in an low-density lipoprotein-based extender and divided into four equal aliquots. Mitochondria-targeted antioxidant (MitoQ) was added at a final concentration of 0 (control), 0.02, 0.2 and 2 μM separately in each aliquotes and cryopreserved. The addition of MitoQ at a concentration of 0.02 μM improved post-thaw sperm motility, plasma membrane integrity and able to sustain sperm motility for a longer time. To investigate MitoQ's effects on mitochondrial function, we measured mitochondrial membrane potential (MMP) using JC-1 dye, superoxide production using Mitosox assay, and lipid peroxidation by TBARS assay. The supplementation of 0.02 μM MitoQ in the extender prevented the significant reduction of MMP and reduced superoxide production resulting in lower lipid peroxidation of sperm plasma membrane after cryopreservation. Further, we found that a higher concentration of MitoQ decreases MMP and increases mitochondrial superoxide production. In conclusion, MitoQ @ 0.02 μM can alleviate oxidative stress by regulating mitochondrial functionality in spermatozoa during cryopreservation.
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