Cloning, functional characterization and evaluating potential in metabolic engineering for lavender (+)-bornyl diphosphate synthase

Abstract The monoterpene (+)-borneol contributes scent and medicinal properties to some plants. It also is the immediate precursor to camphor, another important determinant of aroma and medicinal properties in many plants. (+)-borneol is generated through the dephosphorylation of bornyl diphosphate (BPP), which is itself derived from geranyl diphosphate (GPP) by the enzyme (+)-bornyl diphosphate synthase ((+)-BPPS). In this study we isolated and functionally characterized a novel (+)-BPPS cDNA from Lavandula x intermedia . The cDNA excluding its transit peptide was expressed in E. coli , and the corresponding recombinant protein was purified with Ni–NTA agarose affinity chromatography. The recombinant (+)-LiBPPS catalyzed conversion of GPP to BPP as the major product, and a few minor products. We also investigated the in planta role of (+)-LiBPPS in terpenoid metabolism through its overexpression in sense and antisense orientations in stably transformed Lavandula latifolia plants. The overexpression of (+)-LiBPPS in antisense resulted in reduced production of (+)-borneol and camphor without compromising plant growth and development. As anticipated, the overexpression of the gene led to enhanced production of borneol and camphor, although growth and development were severely impaired in most transgenic lines strongly and ectopically expressing the (+)-LiBPPS transgene in sense. Our results demonstrate that LiBPPS would be useful in studies aimed at the production of recombinant borneol and camphor in vitro , and in metabolic engineering efforts aimed at lowering borneol and camphor production in plants. However, overexpression in sense may require a targeted expression of the gene in glandular trichomes using a trichome-specific promoter.