体内
流式细胞术
内体
磷脂
细胞培养
固体脂质纳米粒
化学
体外
细胞生物学
细胞
原位
生物物理学
共焦显微镜
药物输送
分子生物学
生物化学
生物
膜
有机化学
生物技术
遗传学
作者
Qi Wang,Peifeng Liu,Tao Gong,Xun Sun,Yourong Duan,Zhirong Zhang
标识
DOI:10.1166/jbn.2014.1776
摘要
Drug-phospholipid lipid nanoparticles (DPLNs) can effectively enhance the properties of traditional solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs), as previously demonstrated by our research group and others. To date, however, very few studies have focused on the cellular uptake mechanism and fate of DPLNs in hepatoma. Therefore, we systematically studied the cellular uptake mechanism and endosomal fate of DPLNs through in vitro and in vivo experiments. Confocal laser scanning microscopy (CLSM) and flow cytometry demonstrated that the Raw264.7 cell line (macrophage Raw264.7 cells), Chang cells (a human liver cell line) and HepG2 cells (a human hepatoma cell line) exhibited distinct uptake mechanisms. The Raw264.7 cells served as a model for examining liver-targeting ability. The results from mice with subcutaneous hepatomas and in situ hepatomas confirmed that the liver tumor-targeting property of the DPLNs was associated with the liver drug reservoir function. These findings further improve our understanding of DPLNs for clinical applications.
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