精氨酸
内科学
内分泌学
胰岛素样生长因子
生长因子
鸟氨酸
胰岛素
化学
生物
医学
氨基酸
受体
生物化学
作者
Xiuling Ma,Changjiang Guo,Zhanhui Geng,Jijun Yang,Jingyu Wei,Lanxing Gao
摘要
Objective To explore the regulatory effect of arginine on the secretion of hepatic insulin-like growth factor-1 (IGF-Ⅰ), and the mechanism of enhancing the immune function by arginine. Methods Wistar rats were randomly divided into normal control (NC), wound control (WC), and wound with arginine (Arg) groups, with 8 rats in each group. The rats in WC and Arg groups were inflicted with soft tissue trauma on the back. The rats in Arg group were fed a diet supplemented with 5% arginine for one week, while those in NC and WC groups were fed with glycin. The serum contents of arginine, ornithine, growth factor (GH), NO and IGF-Ⅰ were determined 7 days after feeding. T cell proliferation and IGF-Ⅰ mRNA expression in hepatic tissue were also measured. Meanwhile, the rat hepatocytes were cultured in serum-free medium containing different concentrations of arginine. The supernatant was collected for the determination of IGF-Ⅰ level. Results 1). There was no obvious difference of the serum level of arginine and ornithine between NC and WC groups (P0.05), but the contents of them were obviously higher in the Arg group compared with other two groups (P0.01). 2). No difference in the serum GH level was found among all the groups (P0.05), but the serum NO content in WC and Arg groups was significantly lower than that in NC group (P0.01), and the serum IGF-Ⅰ content in WC group decreased obviously compared with that in NC group (P0.01). 3).The thymocyte proliferation rate in WC group was also markedly lower than that in NC group (P0.01), but that in Arg group was improved compared with WC group (P0.01).4).The expression of hepatic IGF-Ⅰ mRNA: The relative value of IGF-Ⅰ mRNA was 1.19±0.06, 1.08±0.06 and 1.29±0.06 in NC, WC and Arg, respectively, while the value in WC was lower than that in NC (P0.05) group, and that in Arg group was much higher than that in WC group (P0.01). 5). The IGF-Ⅰ level in the supernatant of cultured hepatocytes: When Arg concentration was 0.075 0, 0.750 0,7.500 0 mmol/L in the culture medium, the IGF-Ⅰ level in the supernatant of hepatic cell medi-um was obviously higher than that in the medium without arginine (P0.01). Although IGF-Ⅰ level decreased in the culture medium with arginine in the dose of 37.500 0 mmol/L, it was still obviously higher than that in the medium without arginine (P0.01). Conclusion Arginine could also produce the immune enhancing effect by stimulating hepatic IGF-Ⅰ secretion.
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