乙酰丁酸梭菌
丙酮
丁醇
发酵
化学
生物化学
枯草芽孢杆菌
乙醇
生物
细菌
遗传学
作者
Xiaoning Shen,Dong Liu,Jun Liu,Yanyan Wang,Jiahui Xu,Zhengjiao Yang,Ting Guo,Huanqing Niu,Hanjie Ying
标识
DOI:10.1016/j.biortech.2016.05.121
摘要
Butanol is an important industrial chemical and an attractive transportation fuel. However, the deficiency of reducing equivalents NAD(P)H in butanol fermentation results in a large quantity of oxidation products, which is a major problem limiting the atom economy and economic viability of bio-butanol processes. Here, we integrated the butanol fermentation process with a NADH-generating, acetoin biosynthesis process to improve the butanol production. By overexpressing the α-acetolactate decarboxylase gene alsD from Bacillus subtilis in Clostridium acetobutylicum, acetoin yield was significantly increased at the cost of acetone. After optimization of fermentation conditions, butanol (12.9 g/L), acetoin (6.5 g/L), and ethanol (1.9 g/L) were generated by the recombinant strain, with acetone no more than 1.8 g/L. Thus, both mass yield and product value were greatly improved. This study demonstrates that reducing power compensation is effective to improve the atom economy of butanol fermentation, and provides a novel approach to improve the economic viability of bio-butanol production.
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