Effects of naringin and baicalin combination on the biological activity of human periodontal ligament cells

Objective:To study the effects of naringin and baicalin combination on the biological activity of human periodontal ligament cells(hPDLCs).Methods:hPDLCs were cultured and identified in vitro.The cells were exposed to naringin,baicalin or combination of the both at various concentrations.The proliferation,collagen-Ⅰ protein expression,alkaline phosphatase(ALP)activity and mineralization of hPDLCs were examined by MTT assay,enzyme linked immunosorbent assay and Von Kossa staining.Results:Naringin at 1.0 mg/L combined with baicalin at 0.1 mg/L showed the strongest promotion effects on the proliferation,collagen-Ⅰ protein expression,ALP activity and mineralization of hPDLCs.Conclusion:Naringin and baicalin combination may play a synergistic effect in the bone formation of hPDLCs.