体外
骨髓
巨噬细胞
巨噬细胞集落刺激因子
细胞生物学
化学
生物
免疫学
生物化学
作者
Anaïs Assouvie,Lisa P. Daley‐Bauer,Germain Rousselet
出处
期刊:Methods in molecular biology
日期:2018-01-01
卷期号:: 29-33
被引量:69
标识
DOI:10.1007/978-1-4939-7837-3_3
摘要
Bone marrow-derived macrophages (BMDM) are primary macrophages obtained by in vitro differentiation of bone marrow cells in the presence of macrophage colony-stimulating factor (M-CSF or CSF1). They are easy to obtain in high yields, can be stored by freezing, and can be obtained from genetically modified mice strains. They are therefore widely used as prototypical macrophages for in vitro studies. In this chapter, we present the method for obtaining BMDMs and freezing them.
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