生物
非翻译区
翻译效率
信使核糖核酸
回文序列
基因
终止密码子
谷氨酸棒杆菌
蛋白质生物合成
序列(生物学)
生物化学
遗传学
分子生物学
翻译(生物学)
清脆的
回文
作者
Deng Chen,Xueqin Lv,Jianghua Li,Yanfeng Liu,Guocheng Du,Rodrigo Ledesma‐Amaro,Long Liu
摘要
Abstract In prokaryotic cells, 3′–5′ exonucleases can attenuate messenger RNA (mRNA) directionally from the direction of the 3′–5′ untranslated region (UTR), and thus improving the stability of mRNAs without influencing normal cell growth and metabolism is a key challenge for protein production and metabolic engineering. Herein, we significantly improved mRNA stability by using synthetic repetitive extragenic palindromic (REP) sequences as an effective mRNA stabilizer in two typical prokaryotic microbes, namely, Escherichia coli for the production of cyclodextrin glucosyltransferase (CGTase) and Corynebacterium glutamicum for the production of N ‐acetylglucosamine (GlcNAc). First, we performed a high‐throughput screen to select 4 out of 380 REP sequences generated by randomizing 6 nonconservative bases in the REP sequence designed as the degenerate base “N.” Secondly, the REP sequence was inserted at several different positions after the stop codon of the CGTase‐encoding gene. We found that mRNA stability was improved only when the space between the REP sequence and stop codon was longer than 12 base pairs (bp). Then, by reconstructing the spacer sequence and secondary structure of the REP sequence, a REP sequence with 8 bp in a stem‐loop was obtained, and the CGTase activity increased from 210.6 to 291.5 U/ml. Furthermore, when this REP sequence was added to the 3′‐UTR of glucosamine‐6‐phosphate N ‐acetyltransferase 1 ( GNA1 ), which is a gene encoding a key enzyme GNA1 in the GlcNAc synthesis pathway, the GNA1 activity was increased from 524.8 to 890.7 U/mg, and the GlcNAc titer was increased from 4.1 to 6.0 g/L in C . glutamicum . These findings suggest that the REP sequence plays an important function as an mRNA stabilizer in prokaryotic cells to stabilize its 3′‐terminus of the mRNA by blocking the processing action of the 3′–5′ exonuclease. Overall, this study provides new insight for the high‐efficiency overexpression of target genes and pathway fine‐tuning in bacteria.
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