生物
重组酶
PDGFRA公司
Cre重组酶
命运图
Cre-Lox重组
祖细胞
转基因
计算生物学
细胞生物学
遗传学
转基因小鼠
干细胞
基因
癌症研究
重组
间质细胞
主旨
作者
Ximeng Han,Zhenqian Zhang,Lingjuan He,Huan Zhu,Yan Li,Wenjuan Pu,Maoying Han,Huan Zhao,Kuo Liu,Yi Li,Xiuzhen Huang,Mingjun Zhang,Hengwei Jin,Zan Lv,Juan Tang,Jinjin Wang,Ruilin Sun,Jian Fei,Xueying Tian,Sheng‐Zhong Duan
出处
期刊:Cell Stem Cell
[Elsevier BV]
日期:2021-02-10
卷期号:28 (6): 1160-1176.e7
被引量:104
标识
DOI:10.1016/j.stem.2021.01.007
摘要
The use of the dual recombinase-mediated intersectional genetic approach involving Cre-loxP and Dre-rox has significantly enhanced the precision of in vivo lineage tracing, as well as gene manipulation. However, this approach is limited by the small number of Dre recombinase driver constructs available. Here, we developed more than 70 new intersectional drivers to better target diverse cell lineages. To highlight their applicability, we used these new tools to study the in vivo adipogenic fate of perivascular progenitors, which revealed that PDGFRa+ but not PDGFRa–PDGFRb+ perivascular cells are the endogenous progenitors of adult adipocytes. In addition to lineage tracing, we used members of this new suite of drivers to more specifically knock out genes in complex tissues, such as white adipocytes and lymphatic vessels, that heretofore cannot be selectively targeted by conventional Cre drivers alone. In summary, these new transgenic tools expand the intersectional genetic approach while enhancing its precision.
科研通智能强力驱动
Strongly Powered by AbleSci AI