钙网蛋白
癌症研究
免疫原性细胞死亡
细胞凋亡
医学
化学
程序性细胞死亡
内质网
作者
Dong Yeon Kim,Ayoung Pyo,Miyong Yun,Ramar Thangam,Sung-Hwan You,Ying Zhang,Ye-Rim Jung,Dinh-Huy Nguyen,Akhil Venu,Hyeon Sik Kim,Myung Ha Yoon,Yeongjin Hong,Jung‐Joon Min
标识
DOI:10.2967/jnumed.120.245290
摘要
Surface-exposed calreticulin (ecto-CRT) is a well-known "eat-me" signal exhibited by dying cells that contributes to their recognition and destruction by the immune system. We assessed the use of a CRT-specific binding peptide for imaging ecto-CRT during immunogenic cell death and its utility for early prediction of treatment response. Methods: A synthetic CRT-specific peptide, KLGFFKR (CRTpep), was labeled with fluorescein isothiocyanate or 18F, and the characteristics of ecto-CRT were evaluated in a colon cancer cell line in vitro and in vivo. Results: In vitro flow cytometry, immunofluorescence staining, and in vivo small-animal PET imaging results showed that CRTpep detected preapoptotic cells treated with immunogenic drugs or radiation but not those treated with the nonimmunogenic drug or a nontherapeutic dose of immunogenic drug. Conclusion: The present results indicate that the CRT-specific peptide would enable the prediction of therapeutic response, thereby facilitating early decisions on continuation or discontinuation of immunogenic treatment.
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