A hemicellulose‐bound form of silicon inhibits cadmium ion uptake in rice (Oryza sativa) cells

半纤维素 水稻 化学 细胞壁 生物物理学 细胞培养 运输机 分子生物学 生物化学 生物 基因 纤维素 遗传学 有机化学
作者
Jie Ma,Hongmei Cai,Congwu He,Wei Zhang,Lijun Wang
出处
期刊:New Phytologist [Wiley]
卷期号:206 (3): 1063-1074 被引量:273
标识
DOI:10.1111/nph.13276
摘要

Summary Silicon ( S i) alleviates cadmium ( C d) toxicity in rice ( O ryza sativa ). However, the chemical mechanisms at the single‐cell level are poorly understood. Here, a suspension of rice cells exposed to C d and/or S i treatments was investigated using a combination of plant cell nutritional, molecular biological, and physical techniques including in situ noninvasive microtest technology ( NMT ), polymerase chain reaction ( PCR ), inductively coupled plasma mass spectroscopy ( ICP ‐ MS ), and atomic force microscopy ( AFM ) in Kelvin probe mode ( KPFM ). We found that S i‐accumulating cells had a significantly reduced net Cd 2+ influx, compared with that in Si‐limited cells. PCR analyses of the expression levels of C d and S i transporters in rice cells showed that, when the S i concentration in the medium was increased, expression of the Si transporter gene Low silicon rice 1 ( L si1 ) was up‐regulated, whereas expression of the gene encoding the transporter involved in the transport of C d, Natural resistance‐associated macrophage protein 5 ( N ramp5 ), was down‐regulated. ICP ‐ MS results revealed that 64% of the total S i in the cell walls was bound to hemicellulose constituents following the fractionation of the cell walls, and consequently inhibited C d uptake. Furthermore, AFM in KPFM demonstrated that the heterogeneity of the wall surface potential was higher in cells cultured in the presence of Si than in those cultured in its absence, and was homogenized after the addition of C d. These results suggest that a hemicellulose‐bound form of S i with net negative charges is responsible for inhibition of C d uptake in rice cells by a mechanism of [ S i‐hemicellulose matrix] C d complexation and subsequent co‐deposition.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
tong发布了新的文献求助10
1秒前
眰恦完成签到,获得积分10
2秒前
开心发布了新的文献求助10
4秒前
Hehhhh发布了新的文献求助10
5秒前
6秒前
10秒前
10秒前
14秒前
Hehhhh完成签到,获得积分10
15秒前
潇洒的诗桃应助开心采纳,获得10
16秒前
hsj发布了新的文献求助10
16秒前
天卢完成签到 ,获得积分10
18秒前
小星星完成签到 ,获得积分10
18秒前
沙沙完成签到 ,获得积分10
18秒前
研友_8DAv0L完成签到,获得积分10
18秒前
风趣的灵枫完成签到 ,获得积分10
18秒前
青青河边草完成签到,获得积分10
20秒前
白鸽应助小王子采纳,获得10
23秒前
23秒前
24秒前
hsj完成签到,获得积分20
25秒前
我不会科研关注了科研通微信公众号
26秒前
百浪多息发布了新的文献求助10
28秒前
温暖霸发布了新的文献求助10
28秒前
31秒前
李加威完成签到 ,获得积分10
31秒前
wangayting发布了新的文献求助30
32秒前
33秒前
timemaster666完成签到,获得积分10
33秒前
临床医学研究中心完成签到,获得积分20
33秒前
36秒前
刻苦的元风完成签到 ,获得积分10
36秒前
熊泰山完成签到 ,获得积分10
38秒前
情怀应助英俊的筝采纳,获得10
38秒前
Sudon完成签到 ,获得积分10
39秒前
青山落日秋月春风完成签到,获得积分10
41秒前
41秒前
July完成签到,获得积分10
42秒前
领导范儿应助百浪多息采纳,获得10
43秒前
iii完成签到 ,获得积分10
43秒前
高分求助中
Sustainability in Tides Chemistry 2800
Kinetics of the Esterification Between 2-[(4-hydroxybutoxy)carbonyl] Benzoic Acid with 1,4-Butanediol: Tetrabutyl Orthotitanate as Catalyst 1000
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
Rechtsphilosophie 1000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Handbook of Qualitative Cross-Cultural Research Methods 600
Very-high-order BVD Schemes Using β-variable THINC Method 568
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3137539
求助须知:如何正确求助?哪些是违规求助? 2788516
关于积分的说明 7787054
捐赠科研通 2444818
什么是DOI,文献DOI怎么找? 1300043
科研通“疑难数据库(出版商)”最低求助积分说明 625784
版权声明 601023