破骨细胞
细胞生物学
兰克尔
信号转导
蛋白激酶C
磷脂酶C
化学
细胞凋亡
生物学中的钙
激酶
细胞内
受体
生物
激活剂(遗传学)
生物化学
作者
Romuald Mentaverri,Shozo Yano,Naibedya Chattopadhyay,Laurent Petit,Olga Kifor,Saı̈d Kamel,Ernest F. Terwilliger,Michel Brazier,Edward M. Brown,Romuald Mentaverri,Shozo Yano,Naibedya Chattopadhyay,Laurent Petit,Olga Kifor,Saı̈d Kamel,Ernest F. Terwilliger,Michel Brazier,Edward M. Brown
标识
DOI:10.1096/fj.06-6304fje
摘要
Intracellular transduction pathways that are dependent on activation of the CaR by Ca(o)2+ have been studied extensively in parathyroid and other cell types, and include cytosolic calcium, phospholipases C, A2, and D, protein kinase C isoforms and the cAMP/protein kinase A system. In this study, using bone marrow cells isolated from CaR-/- mice as well as DN-CaR-transfected RAW 264.7 cells, we provide evidence that expression of the CaR plays an important role in osteoclast differentiation. We also establish that activation of the CaR and resultant stimulation of PLC are involved in high Ca(o)2+-induced apoptosis of mature rabbit osteoclasts. Similar to RANKL, Ca(o)2+ (20 mM) appeared to trigger rapid and significant nuclear translocation of NF-kappaB in a CaR- and PLC-dependent manner. In summary, our data suggest that stimulation of the CaR may play a pivotal role in the control of both osteoclast differentiation and apoptosis in the systems studied here through a signaling pathway involving activation of the CaR, phospholipase C, and NF-kappaB.
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