胶束
内吞作用
化学
两亲性
荧光显微镜
共聚物
生物物理学
乙二醇
异硫氰酸荧光素
荧光
荧光素
高分子化学
有机化学
水溶液
生物化学
聚合物
细胞
生物
物理
量子力学
作者
Zhao Zhang,Qianqian Qu,Jinrong Li,Shaobing Zhou
标识
DOI:10.1002/mabi.201300037
摘要
Abstract Fluorescein isothiocyanate (FITC), a fluorescent probe, is coupled to amphiphilic monomethoxy poly(ethylene glycol)‐block‐poly(ε‐caprolactone) (mPEG‐PCL) copolymers. FITC‐labeled mPEG‐PCL copolymers self‐assemble into micelles through the solvent evaporation method. The cellular internalization is examined using fluorescence microscopy on incubation of NIH‐3T3 fibroblasts with micelles or free FITC solution. The effect of the hydrophilic/hydrophobic ratio on the endocytosis mechanisms is evaluated by fluorescence microscopy on culturing of human hepatoblastoma cells and human umbilical vein endothelial cells, individually, mixed with the micelles holding the same parameters including micelle size, shape, and surface charges. magnified image
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