衰老
药理学
传统医学
细胞生物学
化学
生物
医学
作者
Dinghui Liu,Yanming Chen,Yong Liu,Baoshun Hao,Bin Zhou,Lin Wu,Ting Xia,Lin Chen,Wei-Kang Wu,Xiaoxian Qian
出处
期刊:Journal of Cardiovascular Pharmacology
[Ovid Technologies (Wolters Kluwer)]
日期:2012-03-01
卷期号:59 (3): 222-230
被引量:31
标识
DOI:10.1097/fjc.0b013e31823c1d34
摘要
Objective Senescence of endothelial cells has been implicated in endothelial dysfunction and atherogenesis. This study investigated the effects of Rb1, a major ginsenoside in ginseng, on H2O2-induced senescence in primary human umbilical vein endothelial cells (HUVECs). Methods and Results Real-time PCR and Western blot were used to detect the mRNA and protein expression, respectively. H2O2 (40∼100 μmol/L) effectively increased SA-β-gal activity and PAI-1 mRNA levels, two important senescence related biomarkers, in HUVECs, which were dramatically inhibited by Rb1 pre-incubation. Furthermore, Rb1 administration reversed the H2O2-decreased protein and mRNA levels of eNOS and its phosphorylation at Ser-1177, and the increased eNOS phosphorylation at Thr-495. As a result, Rb1 pretreatment restored the NO generation, as assayed by nitrate reductase method. However, pretreatment with L-NAME, a NOS inhibitor, abolished all the inhibitory effects of Rb1 on senescence. Importantly, Rb1 modulated the H2O2-altered caveolin-1 and pAkt, two most important regulators of eNOS expression and activity, in HUVECs. Conclusions We showed that Rb1 effectively protects HUVECs from senescence through eNOS modulation.
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