黑色素瘤
癌症研究
细胞生长
基因沉默
基因敲除
流式细胞术
细胞凋亡
EPH受体A2
细胞周期
小RNA
化学
生物
分子生物学
信号转导
细胞生物学
受体酪氨酸激酶
基因
生物化学
作者
Xiaogang Chen,Yichen Tang,Jianna Yan,Liang Li,Long Jiang,Yuchong Chen
标识
DOI:10.1016/j.jdermsci.2021.08.005
摘要
Circular RNA (circRNA) has been confirmed to play a vital role in melanoma progression.The regulatory function of circ_0062270, a novel circRNA, in melanoma progression is unclear.Relative expression levels of circ_0062270 and microRNA (miR)-331-3p were determined using qRT-PCR. Cell counting kit 8 assay, EdU staining and flow cytometry were used to measure cell proliferation, cell cycle distribution and apoptosis. The protein levels of proliferation, apoptosis and metastasis-related markers, as well as EPH receptor A2 (EPHA2), were tested using western blot analysis. Besides, cell migration and invasion were evaluated using transwell assay. Meanwhile, the interaction between miR-331-3p and circ_0062270 or EPHA2 was confirmed by dual-luciferase reporter assay or RIP assay. Additionally, tumor xenograft models were constructed to investigate the function of circ_0062270 on melanoma tumor growth in vivo.The expression of circ_0062270 was increased in melanoma tissues and cells. Knockdown of circ_0062270 inhibited proliferation, promoted apoptosis, and repressed metastasis in melanoma. Moreover, circ_0062270 could serve as miR-331-3p sponge, and miR-331-3p could target EPHA2. Furthermore, miR-331-3p inhibitor and EPHA2 overexpression reversed the inhibitory effect of circ_0062270 silencing on melanoma progression. In addition, silenced circ_0062270 also could inhibit melanoma tumor growth in vivo.Circ_0062270 accelerated the progression of melanoma through regulating the miR-331-3p/EPHA2 axis, suggesting that circ_0062270 might be a novel potential therapeutic target for melanoma.
科研通智能强力驱动
Strongly Powered by AbleSci AI