适体
微透析
分析物
模块化设计
体内
原位
化学
电化学
纳米技术
组合化学
计算机科学
材料科学
电极
色谱法
生物
有机化学
生物技术
分子生物学
操作系统
物理化学
作者
Philippe Dauphin-Ducharme,Kyle Ploense,Netzahualcóyotl Arroyo-Currás,Tod E. Kippin,Kevin W. Plaxco
标识
DOI:10.1007/978-1-0716-1803-5_25
摘要
The monitoring of specific molecules in the living body has historically required sample removal (e.g., blood draws, microdialysis) followed by analysis via cumbersome, laboratory-bound processes. Those few exceptions to this rule (e.g., glucose, pyruvate, the monoamines) are monitored using "one-off" technologies reliant on the specific enzymatic or redox reactivity of their targets, and thus not generalizable to the measurement of other targets. In response we have developed in vivo electrochemical aptamer-based (E-AB) sensors, a modular, receptor-based measurement technology that is independent of the chemical reactivity of its targets, and thus has the potential to be generalizable to a wide range of analytes. To further the adoption of this in vivo molecular measurement approach by other researchers and to accelerate its ultimate translation to the clinic, we present here our standard protocols for the fabrication and use of intravenous E-AB sensors.
科研通智能强力驱动
Strongly Powered by AbleSci AI