生物
抄写(语言学)
核糖核酸
RNA聚合酶Ⅱ
细胞生物学
非编码RNA
分子生物学
聚腺苷酸
RNA聚合酶Ⅱ全酶
RNA依赖性RNA聚合酶
基因
发起人
遗传学
基因表达
哲学
语言学
作者
Xuefeng Zhu,Xie Xie,Hrishikesh Das,Benedict G. Tan,Yonghong Shi,Ali Al-Behadili,Bradley Peter,Elisa Motori,Sebastian Valenzuela,Viktor Posse,Claes M. Gustafsson,B.M. Hallberg,Maria Falkenberg
出处
期刊:Cell
[Elsevier]
日期:2022-06-01
卷期号:185 (13): 2309-2323.e24
被引量:30
标识
DOI:10.1016/j.cell.2022.05.006
摘要
The mitochondrial genome encodes 13 components of the oxidative phosphorylation system, and altered mitochondrial transcription drives various human pathologies. A polyadenylated, non-coding RNA molecule known as 7S RNA is transcribed from a region immediately downstream of the light strand promoter in mammalian cells, and its levels change rapidly in response to physiological conditions. Here, we report that 7S RNA has a regulatory function, as it controls levels of mitochondrial transcription both in vitro and in cultured human cells. Using cryo-EM, we show that POLRMT dimerization is induced by interactions with 7S RNA. The resulting POLRMT dimer interface sequesters domains necessary for promoter recognition and unwinding, thereby preventing transcription initiation. We propose that the non-coding 7S RNA molecule is a component of a negative feedback loop that regulates mitochondrial transcription in mammalian cells.
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