化学
半抗原
检出限
色谱法
邻苯二甲酸二丁酯
异硫氰酸荧光素
结合
免疫分析
荧光偏振免疫分析
再现性
荧光
抗体
有机化学
免疫学
物理
数学分析
生物
量子力学
数学
作者
Yingshan Chen,Qiyi He,Ding Ren Shen,Zhengyun Jiang,Sergei A. Eremin,Suqing Zhao
出处
期刊:Food Control
[Elsevier]
日期:2019-11-01
卷期号:105: 38-44
被引量:23
标识
DOI:10.1016/j.foodcont.2018.11.052
摘要
To develop a sensitive fluorescence polarization immunoassay (FPIA) for detecting Diisobutyl phthalate (DIBP) in Yoghurt. Five tracers with different lengths of bridge between the hapten molecule and fluorescein isothiocyanate (FITC), fluoresceinthiocarbamyl ethylenediamine (EDF), fluoresceinthiocarbamyl hexylenediamine (HDF), were synthesized and their respective binding responses with anti-DIBP-specific antibody were evaluated. The physicochemical parameters were optimised for the FPIA. The FITC-labelled DIBP conjugate (DIBP-FITC) was found to be the optimal tracer for FPIA of Diisobutyl phthalate. The detection limit of DIBP, IC50 value and the working range were 0.82, 9.32 and 1.16–74.97 ng/mL, respectively; and the reaction time was only 5min. The average recovery from spiked Yoghurt samples was 77.80–115.60% which showed good reproducibility for FPIA. Thus, the developed FPIA method exhibited the potential for the rapid and accurate determination of DIBP in agricultural and environmental samples.
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