MnO2 switch-bridged DNA walker for ultrasensitive sensing of cholinesterase activity and organophosphorus pesticides

检出限 化学 DNA 有机磷 水解 荧光 胆碱酯酶 杀虫剂 组合化学 色谱法 生物化学 药理学 生物 农学 量子力学 物理
作者
Wei Li,Yuchen Rong,Jingyi Wang,Tianzi Li,Zhenguang Wang
出处
期刊:Biosensors and Bioelectronics [Elsevier BV]
卷期号:169: 112605-112605 被引量:51
标识
DOI:10.1016/j.bios.2020.112605
摘要

Cholinesterases (ChEs) are important indicators of neurological disease, hepatocellular carcinoma, and organophosphate poisoning. In this work, a MnO2 switch-bridged DNA walker was developed for ultrasensitive sensing of ChEs activity. The fuel strands loaded MnO2 switch was designed to bridge the hydrolysis activity of ChEs and the running of the DNA walker. Under the action of ChE, the substrate butyrylcholine is first catalytically hydrolyzed to thiocholine, which then mediates MnO2 nanosheet reduction to Mn2+, releasing the fuel strands into solution. The fuel strands as substitute targets then trigger the continuous operation of DNA walker with the aid of Mn2+, generating detectable fluorescence responses. The detection of ChE activity is converted to DNA detection in this method. Benefited from the robust operation and amplification effect of DNA walker, a wide linear range between the BChE activity and fluorescence intensity of nearly six orders of magnitude (1000–0.005 U/mL) and a limit of detection as low as 0.0008 U/mL are achieved. This allows the direct determination of BChE activity in clinical serum samples without any pretreatments. Moreover, the proposed method has remarkable capabilities for inhibitor (organophosphorus pesticide) screening and quantification, and organophosphorus pesticide detection in real samples is also achieved. Therefore, the MnO2 switch-bridged DNA walker represents a powerful tool for ultrasensitive sensing of ChEs and organophosphorus pesticides, and has great application potential in clinical diagnosis, therapeutics, and drug screening.
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