原噬菌体
精子
生物
细胞生物学
核糖核酸
染色质
组蛋白
遗传学
DNA
精子发生
核糖核酸酶
鱼精蛋白
DNA损伤
分子生物学
基因
生物化学
大肠杆菌
噬菌体
肝素
作者
Rupinder Kaur,Angelina McGarry,J. Dylan Shropshire,Brittany A. Leigh,Seth R. Bordenstein
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2024-03-08
卷期号:383 (6687): 1111-1117
被引量:2
标识
DOI:10.1126/science.adk9469
摘要
The extent to which prophage proteins interact with eukaryotic macromolecules is largely unknown. In this work, we show that cytoplasmic incompatibility factor A (CifA) and B (CifB) proteins, encoded by prophage WO of the endosymbiont Wolbachia, alter long noncoding RNA (lncRNA) and DNA during Drosophila sperm development to establish a paternal-effect embryonic lethality known as cytoplasmic incompatibility (CI). CifA is a ribonuclease (RNase) that depletes a spermatocyte lncRNA important for the histone-to-protamine transition of spermiogenesis. Both CifA and CifB are deoxyribonucleases (DNases) that elevate DNA damage in late spermiogenesis. lncRNA knockdown enhances CI, and mutagenesis links lncRNA depletion and subsequent sperm chromatin integrity changes to embryonic DNA damage and CI. Hence, prophage proteins interact with eukaryotic macromolecules during gametogenesis to create a symbiosis that is fundamental to insect evolution and vector control.
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