沙门氏菌
生物膜
转录组
化学
精氨酸
生物合成
肠沙门氏菌
生物化学
微生物学
基因
生物
基因表达
食品科学
氨基酸
细菌
大肠杆菌
遗传学
作者
Yunge Liu,Yuqing Yan,Ke Yang,Xiaoyin Yang,Pengcheng Dong,Hao Wu,Xin Luo,Yimin Zhang,Lixian Zhu
出处
期刊:Food Control
[Elsevier]
日期:2023-08-01
卷期号:150: 109734-109734
被引量:4
标识
DOI:10.1016/j.foodcont.2023.109734
摘要
The present study aimed to investigate the inhibition of clove essential oil (CEO) and oregano essential oil (OEO) on Salmonella Derby biofilm formation and explore the molecular mechanisms involved in the effects of CEO and OEO by using the transcriptomic approach. The results showed that CEO and OEO significantly inhibited the biofilm formation of S. Derby at both 1/2 and 1/4 MICs, and there was no significant difference in biofilm inhibition between the two sub-MICs. The transcriptomic analysis revealed that 1/4 MIC CEO and OEO (0.2 mg/mL and 0.05 mg/mL) significantly altered gene expression in S. Derby biofilm. Compared with the untreated biofilms, there were 392 differentially expressed genes (DEGs) were up-regulated and 373 DEGs were down-regulated in CEO treatment group, while 679 up-regulated and 709 down-regulated DEGs in OEO treatment group. Interestingly, enrichment analysis of the DEGs showed that both CEO and OEO significantly down-regulated the energy metabolism-related genes and these DEGs were mainly involved in pathways of fatty acid degradation, TCA cycle, and oxidative phosphorylation. This finding was confirmed in subsequent ATP content, ATPase activity and NADH oxidase activity assays. Moreover, compared to OEO treatment, CEO treatment also down-regulated DEGs enriched in ribosome and arginine, lysine, phenylalanine, tyrosine, and tryptophan biosynthesis pathways. This study reveals the biofilm inhibitory effects and mechanisms of CEO and OEO against S. Derby at sub-MICs, which may provide theoretical support for preventing biofilms of foodborne pathogens in the food industry.
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