Jurkat细胞
CD8型
T细胞
细胞毒性T细胞
分子生物学
生物
化学
细胞生物学
免疫学
免疫系统
生物化学
体外
作者
Miao-Ming Yan,Zhao-Xuan Li,Chong Chen,Wei Zhang,Daobin Zhou
出处
期刊:PubMed
日期:2023-02-01
卷期号:31 (1): 71-75
被引量:1
标识
DOI:10.19746/j.cnki.issn.1009-2137.2023.01.011
摘要
To explore the regulatory effect of chidamide on CD8+ T cells in T-cell acute lymphoblastic leukemia.The expression levels of CXCL9 and CXCL3 mRNA in Jurkat cells, lymphocytes treated with chidamide and lymphocytes co-cultured with chidamide-treated Jurkat cells were detected by fluorescence quantitative PCR. The proportion of CD8+ T cells in lymphocytes treated with chidamide and lymphocytes co-cultured with chidamide-treated Jurkat cells was determined by flow cytometry.Chidamide upregulated CXCL9 mRNA expression in Jurkat cell line in a dose-dependent manner (r=0.950). The mRNA expression of CXCL9 in chidamide 5 μmol/L group was 164 times higher than that in control group. Chidamide upregulated CXCL9 mRNA expression in lymphocytes, but the up-regulated level was significantly lower than that in Jurkat cell line treated with the same concentration of chidamide. Co-culture with chidamide treated Jurkat cells upregulated the proportion of CD8+ T cells in lymphocytes.In T-cell acute lymphoblastic leukemia, chidamide may increase the concentration of CXCL9 in the tumor microenvironment by up-regulating the expression of CXCL9 in tumor cells, leading to an increase in the number of CD8+ T cells.西达本胺对急性T淋巴细胞白血病CD8+ T细胞的 调控作用研究.探讨西达本胺对急性T淋巴细胞白血病CD8+T细胞的调控作用.通过荧光定量PCR检测西达本胺处理的Jurkat细胞、淋巴细胞、与西达本胺处理的Jurkat细胞共培养的淋巴细胞的CXCL9、CXCL3 mRNA表达水平。通过流式细胞术检测西达本胺处理的淋巴细胞、与西达本胺处理的Jurkat细胞共培养的淋巴细胞中CD8+T细胞的比 例.西达本胺上调Jurkat细胞系CXCL9的mRNA表达,上调水平呈剂量依赖(r=0.950),西达本胺5 μmol/L 组的CXCL9 mRNA表达是无处理组的164倍。西达本胺上调淋巴细胞CXCL9的mRNA表达,上调水平明显低于同浓度西达本胺处理的Jurkat细胞系。与西达本胺处理的Jurkat细胞共培养,可以上调淋巴细胞中CD8+T细胞的比例.急性T淋巴细胞白血病中,西达本胺可能通过上调肿瘤细胞CXCL9的表达,增加肿瘤微环境中CXCL9的浓度,导致CD8+T细胞数量的增加.
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