Amphiphilic Lysine-based Glycopeptides Exert Antibacterial Effect on Pseudomonas aeruginosa

The development of new antibiotics with novel antimicrobial mechanisms and strategies has attracted considerable interest. Herein, three amphiphilic lysine-based glycopeptides (SA-l-Gal, OA-l-Gal and LOA-l-Gal) were developed, and their antibacterial activity and inhibition effects on biofilm formation on P. aeruginosa were studied. SA-l-Gal with a stearic acid group modification exhibited better antibacterial effects than the other lysine-based glycopeptides by damaging the bacterial membrane. Furthermore, SA-l-Gal could effectively reduce inflammation factor expression, enhance the formation of new blood vessels, and promote the healing of P. aeruginosa-infected mouse wounds. This result provides insights into the development of glycomimetic drugs against P. aeruginosa infection.