Unveiling the impact of flooding and salinity on iron oxides-mediated binding of organic carbon in the rhizosphere of Scirpus mariqueter

The abundant Fe (hydr-) oxides present in wetland sediments can form stable iron (Fe)-organic carbon (OC) complexes (Fe-OC), which are key mechanisms contributing to the stability of sedimentary OC stocks in coastal wetland ecosystems. However, the effects of increased flooding and salinity stress, resulting from global change, on the Fe-OC complexes in sediments remain unclear. In this study, we conducted controlled experiments in a climate chamber to quantify the impacts of flooding and salinity on the different forms of Fe (hydr-) oxides binding to OC in the rhizosphere sediments of S. mariqueter as well as the influence on Fe redox cycling bacteria in the rhizosphere. The results of this study demonstrated that prolonged flooding and high salinity treatments significantly reduced the content of organo-metal complexes (FePP) in the rhizosphere. Under high salinity conditions, the content of FePP-OC increased significantly, while flooding led to a decrease in FePP-OC content, inhibiting co-precipitation processes. The association of amorphous Fe (hydr-) oxides (FeHH) with OC showed no significant differences under different flooding and salinity treatments. Prolonged flooding significantly increased the relative abundance of Fe-reducing bacteria (FeRB) Deferrisoma and Geothermobacter and decreased polyphenol oxidase in the rhizosphere, while the relative abundance of Fe-oxidizing bacteria (FeOB) Paracoccus and Pseudomonas decreased with increasing salinity and duration of flooding. Overall, short-term water and salinity stress promoted the binding of FeDH to OC in the rhizosphere of S. mariqueter, leading to a reduction in the OC content held by FePP. However, there were no significant differences observed in the OC stocks or the total Fe-OC content in the rhizosphere sediments. The findings suggest a degree of consistency in the Fe-OC of the "plant-soil" complex system within tidal flat wetlands, showing resilience to abrupt shifts in flooding and salinity over short periods.