硫酸化
糖胺聚糖
硫酸软骨素
生物化学
硫酸乙酰肝素
多糖
硫酸可拉坦
生物
化学
作者
Daoan Wang,Litao Hu,Ruirui Xu,Weijiao Zhang,Haibo Xiong,Yang Wang,Guocheng Du,Zhen Kang
标识
DOI:10.1016/j.enzmictec.2023.110324
摘要
Glycosaminoglycans (GAGs) are naturally occurring acidic polysaccharides with wide applications in pharmaceuticals, cosmetics, and health foods. The diverse biological activities and physiological functions of GAGs are closely associated with their molecular weights and sulfation patterns. Except for the non-sulfated hyaluronan which can be synthesized naturally by group A Streptococcus, all the other GAGs such as heparin and chondroitin sulfate are mainly acquired from animal tissues. Microbial cell factories provide a more effective platform for the production of structurally homogeneous GAGs. Enhancing the production efficiency of polysaccharides, accurately regulating the GAGs molecular weight, and effectively controlling the sulfation degree of GAGs represent the major challenges of developing GAGs microbial cell factories. Several enzymatic, metabolic engineering, and synthetic biology strategies have been developed to tackle these obstacles and push forward the industrialization of biotechnologically produced GAGs. This review summarizes the recent advances in the construction of GAGs synthesis cell factories, regulation of GAG molecular weight, and modification of GAGs chains. Furthermore, the challenges and prospects for future research in this field are also discussed.
科研通智能强力驱动
Strongly Powered by AbleSci AI