后代
氧化应激
苯并(a)芘
细胞凋亡
生殖细胞
组蛋白
男科
怀孕
化学
生物
致癌物
遗传学
内分泌学
医学
基因
作者
Lin Zhang,Wenqi Chen,Xiaoying Han,Li Wang,Pingjin Gao,Xinran Ma,Zheng Cao,Changhua Sun,Dong Cheng,Jing Wang,Qilong He,Shuzhen Liu
标识
DOI:10.1016/j.scitotenv.2024.175877
摘要
Infertility has gradually become a global health concern, and evidence suggests that exposure to environmental endocrine-disrupting chemicals (EDCs) represent one of the key causes of infertility. Benzo(a)pyrene (BaP) is a typical EDC that is widespread in the environment. Previous studies have detected BaP in human urine, semen, cervical mucus, oocytes and follicular fluid, resulting in reduced fertility and irreversible reproductive damage. However, the mechanisms underlying the effects of gestational BaP exposure on offspring fertility in male mice have not been fully explored. In this study, pregnant mice were administered BaP at doses of 0, 5, 10 and 20 mg/kg/day via gavage from Days 7.5 to 12.5 of gestation. The results revealed that BaP exposure during pregnancy disrupted the structural integrity of testicular tissue, causing a disorganized arrangement of spermatogenic cells, compromised sperm quality, elevated levels of histone modifications and increased apoptosis in the testicular tissue of F1 male mice. Furthermore, oxidative stress was also increased in the testicular tissue of F1 male mice. BaP activates the AhR/ERα signaling pathway, affects H3K4me3 expression and induces apoptosis in testicular tissue. AhR and Cyp1a1 are overexpressed, and the expression of key molecules in the antioxidant pathway, including Keap1 and Nrf2, is reduced. The combined effects of these molecules lead to apoptosis in testicular tissues, damaging and compromising sperm quality. This impairment in testicular cells further contributes to compromised testicular tissues, ultimately impacting the reproductive health of F1 male mice.
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