类胡萝卜素
脱落酸
成熟
叶黄素
生物合成
生物
生物化学
染色体体
烟草
质体
烟草
转基因番茄
植物
基因
转基因
转基因作物
叶绿体
作者
Yan Yu,Zeyang Bao,Qihang Zhou,Wei Wu,Wei Chen,Zhenfeng Yang,Li Wang,Xuewen Li,Shifeng Cao,Liyu Shi
出处
期刊:Foods
[MDPI AG]
日期:2024-09-06
卷期号:13 (17): 2829-2829
标识
DOI:10.3390/foods13172829
摘要
The yellow-fleshed loquat is abundant in carotenoids, which determine the fruit’s color, provide vitamin A, and offer anti-inflammatory and anti-cancer health benefits. In this research, the impact of abscisic acid (ABA), a plant hormone, on carotenoid metabolism and flesh pigmentation in ripening loquat fruits was determined. Results revealed that ABA treatment enhanced the overall content of carotenoids in loquat fruit, including major components like β-cryptoxanthin, lutein, and β-carotene, linked to the upregulation of most genes in the carotenoid biosynthesis pathway. Furthermore, a transcription factor, EjWRKY6, whose expression was induced by ABA, was identified and was thought to play a role in ABA-induced carotenoid acceleration. Transient overexpression of EjWRKY6 in Nicotiana benthamiana and stable genetic transformation in Nicotiana tabacum with EjWRKY6 indicated that both carotenoid production and genes related to carotenoid biosynthesis could be upregulated in transgenic plants. A dual-luciferase assay proposed a probable transcriptional control between EjWRKY6 and promoters of genes associated with carotenoid production. To sum up, pre-harvest ABA application could lead to carotenoid biosynthesis in loquat fruit through the EjWRKY6-induced carotenoid biosynthesis pathway.
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